Human Free BDNF ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DBD00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL), Citrate Plasma (50 µL)
Sensitivity
20 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
Product Summary for Human Free BDNF Quantikine ELISA Kit
The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human BDNF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human free BDNF in this assay. No medical histories were available for the donors used in
this study.
*Samples were diluted prior to assay.
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum* (n=33) | 27,793 | 100 | 6186-42,580 |
| Platelet-poor EDTA plasma (n=33) | 428 | 56 | ND-4137 |
| Platelet-poor heparin plasma (n=33) | 196 | 55 | ND-528 |
| Platelet-poor citrate plasma (n=33) | 401 | 94 | ND-1838 |
ND=Non-detectable
Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106
cells/mL) were
cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate and stimulated
with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 1 and 5 and
assayed for levels of human free BDNF.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 132 | 109 |
| Stimulated | 160 | 104 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 334 | 825 | 1339 | 358 | 905 | 1517 |
| Standard Deviation | 15.6 | 28.3 | 56.5 | 36.8 | 86.8 | 121.6 |
| CV% | 4.7 | 3.4 | 4.2 | 10.3 | 9.6 | 8.1 |
Citrate Plasma, EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 476 | 1258 | 2175 | 528 | 1338 | 2287 |
| Standard Deviation | 24.0 | 48.2 | 135.7 | 59.7 | 102.3 | 186.0 |
| CV% | 5.0 | 3.8 | 6.2 | 11.3 | 7.6 | 8.1 |
Recovery for Human Free BDNF Quantikine ELISA Kit
The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 96 | 83-105 |
| Platelet-poor Citrate Plasma (n=5) | 99 | 87-120 |
| Platelet-poor EDTA Plasma (n=5) | 96 | 89-115 |
| Platelet-poor Heparin Plasma (n=5) | 93 | 81-119 |
| Serum (n=5) | 100 | 85-116 |
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Free BDNF Quantikine ELISA Kit
Human Free BDNF ELISA Cell Culture Supernate Standard Curve
Human Free BDNF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BDNF
Long Name
Brain-derived Neurotrophic Factor
Alternate Names
Abrineurin, ANON2, BULN2, Neurotrophin
Gene Symbol
BDNF
Additional BDNF Products
Product Documents for Human Free BDNF Quantikine ELISA Kit
Product Specific Notices for Human Free BDNF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.