Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

Name: Human Free IGFBP-1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DGB100

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human Free IGFBP-1 Quantikine ELISA Kit

R&D Systems, part of Bio-Techne | Catalog # DGB100

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL), Human Milk (10 µL)

Sensitivity

13.8 pg/mL

Assay Range

125-8000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)

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Product Summary for Human Free IGFBP-1 Quantikine ELISA Kit

The Quantikine Human Free IGFBP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human free IGFBP-1 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human IGFBP-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human free IGFBP-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant phosphorylated and non-phosphorylated human free IGFBP-1. This assay does not detect IGFBP-1 in complex with IGF-I or IGF-II.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1038	3065	5639	1079	3100	5895
Standard Deviation	58.2	131	230	103	209	373
CV%	5.6	4.3	4.1	9.5	6.7	6.3

Recovery for Human Free IGFBP-1 Quantikine ELISA Kit

The recovery of human Free IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	112	92-117
Cell Lysates (n=4)	107	91-117
EDTA Plasma (n=4)	97	86-111
Heparin Plasma (n=4)	97	85-108
Human Milk (n=4)	104	92-114
Serum (n=4)	94	86-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Free IGFBP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human Free IGFBP-1 Quantikine ELISA Kit

Human Free IGFBP-1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-1

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name

Insulin-like Growth Factor Binding Protein 1

Alternate Names

IGFBP1

Entrez Gene IDs

3484 (Human); 16006 (Mouse)

Gene Symbol