Human GDF-15 Quantikine QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK957
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (10 µL)
Sensitivity
0.947 pg/mL
Assay Range
23.4-1500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human GDF-15 Quantikine QuicKit ELISA
The Quantikine™ QuicKit™ Human GDF-15 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human GDF-15 levels in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and antibodies raised against the recombinant protein. Results obtained using natural human GDF-15 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human GDF-15.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Recognizes natural and recombinant human GDF-15
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human GDF-15 in this assay. No medical histories were available for the donors
used in this study.
Cell Culture Supernates:
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=10) | 556 | 204-1146 | 315 |
| EDTA plasma (n=10) | 511 | 249-1042 | 281 |
| Heparin plasma (n=10) | 539 | 243-1009 | 282 |
| Urine (n=4) | 4783 | 3243-8287 | 2353 |
Cell Culture Supernates:
HepG2 cells were cultured in MEM NEAA Earle's Salts supplemented with 10% fetal bovine
serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until
nearly confluent. The cell conditioned media was taken off and centrifuged to remove any cells
or debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed
for human GDF-15, and measured at 47,216 pg/mL.
JAR cells were cultured in DMEM NEAA Earle's Salts supplemented with 10% FBS, 2 mM
L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly confluent.
The cell conditioned media was taken off and centrifuged to remove any cells or debris and
stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for human
GDF-15, and measured at 29,417 pg/mL.
JEG-3 cells were cultured in MEM NEAA Earle's Salts supplemented with 10% FBS,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly
confluent. The cell conditioned media was taken off and centrifuged to remove any cells or
debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for
human GDF-15, and measured at 5651 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 156 | 882 | 165 | 925 |
| Standard Deviation | 6.12 | 58.8 | 9.49 | 77.6 |
| CV% | 3.9 | 6.7 | 5.7 | 8.4 |
Recovery for Human GDF-15 Quantikine QuicKit ELISA
The recovery of human GDF-15 spiked to three levels in samples throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 92-113 |
| EDTA Plasma (n=2) | 74 | 66-83 |
| Heparin Plasma (n=2) | 77 | 66-93 |
| Serum (n=2) | 77 | 66-84 |
| Urine (n=2) | 104 | 94-116 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human GDF-15 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human GDF-15 Quantikine QuicKit ELISA
Human GDF-15 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-15
Long Name
Growth Differentiation Factor 15
Alternate Names
GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta
Gene Symbol
GDF15
Additional GDF-15 Products
Product Documents for Human GDF-15 Quantikine QuicKit ELISA
Product Specific Notices for Human GDF-15 Quantikine QuicKit ELISA
For research use only
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