Human GM-CSF Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HSGM0
Key Product Details
Assay Length
6.5 hours
Sample Type & Volume Required Per Well
Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL), Urine (200 µL)
Sensitivity
0.26 pg/mL
Assay Range
1-64 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
0.5-32 pg/mL (Urine)
0.5-32 pg/mL (Urine)
Product Summary for Human GM-CSF Quantikine HS ELISA Kit
The Quantikine HS Human GM-CSF Immunoassay is a 6.5 hour solid phase ELISA designed to measure human GM-CSF in serum, plasma, and urine. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GM-CSF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 18 | 18 | 18 |
| Mean (pg/mL) | 3.68 | 22.0 | 44.6 | 2.8 | 16.2 | 33.2 |
| Standard Deviation | 0.35 | 1.07 | 1.76 | 0.66 | 2.47 | 4.6 |
| CV% | 9.5 | 4.9 | 4.0 | 23.5 | 15.3 | 13.9 |
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 2.69 | 7.05 | 24.8 | 3.77 | 14.3 | 27.4 |
| Standard Deviation | 0.14 | 0.33 | 0.73 | 0.37 | 1.17 | 1.85 |
| CV% | 5.2 | 4.7 | 2.9 | 9.8 | 8.2 | 6.8 |
Recovery for Human GM-CSF Quantikine HS ELISA Kit
The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 114 | 88-140 |
| Heparin Plasma (n=4) | 109 | 91-122 |
| Serum (n=4) | 109 | 94-128 |
| Urine (n=5) | 102 | 95-116 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human GM-CSF Quantikine HS ELISA Kit
Human GM-CSF ELISA Urine Assay
Human GM-CSF ELISA Serum/Plasma Assay
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Entrez Gene IDs
Gene Symbol
CSF2
Additional GM-CSF Products
Product Documents for Human GM-CSF Quantikine HS ELISA Kit
Product Specific Notices for Human GM-CSF Quantikine HS ELISA Kit
For research use only
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