Human Granzyme B DuoSet ELISA Best Seller
R&D Systems, part of Bio-Techne | Catalog # DY2906-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
39.1-2500 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Granzyme B DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Granzyme B DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Granzyme B. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Granzyme B DuoSet ELISA
Human Granzyme B ELISA Standard Curve
Kit Contents for Human Granzyme B DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Granzyme B
As one of the five Granzymes (A, B, H, K and M) identified in the human genome, Granzyme B
(32kDa) (5) is the most widely researched in terms of its biological function and its utility in
health and disease (4). It is synthesized as a precursor (247 residues) with a signal peptide
(residues 1-18), a pro-peptide (residues 19-20), and a mature chain (residues 21-247) (6-8).
Once inside granules, Granzyme B is fully processed into the mature chain and becomes an
active protease when the pro-peptide, Gly-Glu is removed from the N-terminus by cleavage
with Cathepsin C (9). The protease activity of Granzyme B is tightly controlled by Serpin B9/
Protease Inhibitor 9 (9). The amino acid sequence of human Granzyme B is 71%, 69%, and 68%
identical to its canine, rat, and mouse counterparts, respectively.
Granzymes have been shown to modulate inflammation, and Granzyme B plasma levels have
been found higher with atopic dermatitis and psoriasis when compared to healthy controls.
This is in contrast to Granzyme A plasma levels which remain unchanged (10). Serum from
patients with Crohn's disease have significantly higher Granzyme B levels than controls (11).
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
Additional Granzyme B Products
Product Documents for Human Granzyme B DuoSet ELISA
Product Specific Notices for Human Granzyme B DuoSet ELISA
For research use only
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