Human Growth Hormone Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGH00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
7.18 pg/mL
Assay Range
25-1600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Growth Hormone Quantikine ELISA Kit
The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Growth Hormone
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human Growth Hormone in this assay. No medical histories were available for the donors
used in this study.
ND=Non-detectable
| Females | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=18) | 550 | 100 | 29.3-4360 |
| EDTA plasma (n=18) | 648 | 100 | 30.2-5936 |
| Heparin plasma (n=18) | 641 | 100 | 38.1-5392 |
| Males | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=17) | 62.0 | 83 | ND-120.5 |
| EDTA plasma (n=17) | 56.4 | 86 | ND-99.2 |
| Heparin plasma (n=17) | 58.1 | 86 | ND-109.6 |
Cell culture Supernates - - Human peripheral blood cells (1 x 106
cells/mL) were cultured in
DMEM supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were
cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates
were removed and assayed for levels of human Growth Hormone. No detectable levels were
observed.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 201 | 595 | 1091 | 209 | 623 | 1152 |
| Standard Deviation | 9.4 | 18.8 | 26.0 | 18.2 | 45.6 | 63.6 |
| CV% | 4.7 | 3.2 | 2.4 | 8.7 | 7.3 | 5.5 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 197 | 586 | 1092 | 215 | 639 | 1189 |
| Standard Deviation | 8.0 | 13.8 | 29.8 | 20.2 | 49.5 | 82.2 |
| CV% | 4.1 | 2.4 | 2.7 | 9.4 | 7.7 | 6.9 |
Recovery for Human Growth Hormone Quantikine ELISA Kit
The recovery of Growth Hormone spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 97-105 |
| EDTA Plasma (n=4) | 108 | 101-117 |
| Heparin Plasma (n=4) | 107 | 99-117 |
| Serum (n=4) | 105 | 98-111 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Growth Hormone were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Growth Hormone Quantikine ELISA Kit
Human Growth Hormone ELISA Calibrator Diluent RD5K Standard Curve
Human Growth Hormone ELISA Calibrator Diluent RD6-15 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Growth Hormone
Alternate Names
GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin
Entrez Gene IDs
2688 (Human)
Gene Symbol
GH1
Additional Growth Hormone Products
Product Documents for Human Growth Hormone Quantikine ELISA Kit
Product Specific Notices for Human Growth Hormone Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.