Human Haptoglobin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DHAPG0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (20 µL)
Sensitivity
0.529 ng/mL
Assay Range
3.13-200 ng/mL (Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human Haptoglobin Quantikine ELISA Kit
The Quantikine Human Haptoglobin Immunoassay is a 4.5 hour solid phase ELISA
designed to measure haptoglobin in serum-free cell culture supernates, serum, plasma, saliva, and urine
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human haptoglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
EDTA Plasma, Heparin Plasma, Saliva, Serum, Serum-free Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 18.7 | 58.3 | 116 | 18.8 | 57.8 | 113 |
| Standard Deviation | 0.690 | 1.42 | 3.66 | 1.40 | 3.47 | 6.30 |
| CV% | 3.7 | 2.4 | 3.2 | 7.4 | 6.0 | 5.6 |
Recovery for Human Haptoglobin Quantikine ELISA Kit
The recovery of human haptoglobin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Serum-free Cell Culture Media (n=4) | 101 | 94-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human haptoglobin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Haptoglobin Quantikine ELISA Kit
Human Haptoglobin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Haptoglobin
Alternate Names
HP, HP-1, hp2-alpha, HPA1S
Gene Symbol
HP
Additional Haptoglobin Products
Product Documents for Human Haptoglobin Quantikine ELISA Kit
Product Specific Notices for Human Haptoglobin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.