Human HSP90 beta DuoSet ELISA

Name: Human HSP90 beta DuoSet ELISA
Brand: R&D Systems
SKU: DY32861-05

R&D Systems, part of Bio-Techne | Catalog # DY32861-05

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Product Datasheet / COA / SDS

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	0.625 - 40 ng/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human HSP90 beta DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human HSP90 beta DuoSet ELISA

Technical Data
Citations / Reviews

Summary for Human HSP90 beta DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human HSP90beta. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human HSP90 beta DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human HSP90 beta DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (R&D Systems,

Catalog #DY999B)

Stop Solution:2N H2SO4(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990)

Plate Sealers:ELISA Plate Sealers (Catalog #DY992)

*For the Reagent Diluent and Blocking Buffer arerecommended for a specific DuoSet ELISA Development Kit,see the product datasheet.

Scientific Data Examples for Human HSP90 beta DuoSet ELISA

	Human HSP90 beta DuoSet ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HSP90 beta

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Item	Value
Long Name	Heat Shock Protein 90 beta
Entrez Gene IDs	3326 (Human)
Alternate Names	D6S182, HSP 84, HSP84, HSP90 beta, HSP90-BETA, HSP90AB1, HSP90B, HSP90BHSP 90, HSPC2, HSPC2D6S182, HSPCB, HSPCBFLJ26984, Heat shock 84 kDa, heat shock 90kD protein 1, beta, heat shock 90kDa protein 1, beta, heat shock protein 90kDa alpha (cytosolic), class B member 1, heat shock protein HSP 90-beta, heat shock protein beta

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