Human IGF-II/IGF2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DG200
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)
Sensitivity
5.41 pg/mL
Assay Range
12.5-800 pg/mL (Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human IGF-II/IGF2 Quantikine ELISA Kit
The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum, Serum-free Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 128 | 305 | 566 | 136 | 307 | 558 |
| Standard Deviation | 4.05 | 7.20 | 15.6 | 9.89 | 28.5 | 28.2 |
| CV% | 3.2 | 2.4 | 2.8 | 7.3 | 9.3 | 5.1 |
Recovery for Human IGF-II/IGF2 Quantikine ELISA Kit
The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Serum-free Cell Culture Media (n=4) | 95 | 82-104 |
| Urine (n=4) | 94 | 79-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IGF-II/IGF2 Quantikine ELISA Kit
Human IGF-II ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-II/IGF2
Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.
The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
Long Name
Insulin-like Growth Factor II
Alternate Names
C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974
Gene Symbol
IGF2
Additional IGF-II/IGF2 Products
Product Documents for Human IGF-II/IGF2 Quantikine ELISA Kit
Product Specific Notices for Human IGF-II/IGF2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.