Human IL-18 BPa Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DBP180
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
Sensitivity
7.52 pg/mL
Assay Range
26.6-1700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human IL-18 BPa Quantikine ELISA Kit
The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA
designed to measure IL-18 BPa in cell culture supernates, cell lysates,
serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant human IL-18 BPa.
Results obtained using natural human IL-18 BPa showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural human IL-18 BPa.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-18 BPa
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 123 | 404 | 789 | 136 | 434 | 841 |
| Standard Deviation | 5.50 | 9.49 | 21.1 | 9.47 | 32.8 | 59.1 |
| CV% | 4.5 | 2.3 | 2.7 | 7.0 | 7.6 | 7.0 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 109 | 368 | 740 | 115 | 362 | 692 |
| Standard Deviation | 5.73 | 16.4 | 15.8 | 13.5 | 29.1 | 48.3 |
| CV% | 5.3 | 4.5 | 2.1 | 11.7 | 8.0 | 7.0 |
Recovery for Human IL-18 BPa Quantikine ELISA Kit
The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 106 | 98-114 |
| EDTA Plasma (n=4) | 106 | 93-113 |
| Heparin Plasma (n=4) | 105 | 92-119 |
| Serum (n=4) | 102 | 92-113 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-18 BPa Quantikine ELISA Kit
Human IL-18 BPa ELISA Calibrator Diluent RD5-18 Standard Curve
Human IL-18 BPa ELISA Calibrator Diluent RD6-68 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-18 BPa
Long Name
Interleukin 18 Binding Protein Isoform a
Alternate Names
IL18 BPa, IL18BP
Gene Symbol
IL18BP
Additional IL-18 BPa Products
Product Documents for Human IL-18 BPa Quantikine ELISA Kit
Product Specific Notices for Human IL-18 BPa Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.