Human IL-3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # D3000
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
7.4 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-3 Quantikine ELISA Kit
The Quantikine Human IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-3 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 126 | 731 | 991 | 128 | 725 | 1423 |
| Standard Deviation | 5.7 | 23.7 | 30.4 | 10.1 | 45.9 | 81.5 |
| CV% | 4.5 | 3.2 | 3.1 | 7.9 | 6.3 | 5.7 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 115 | 728 | 1460 | 124 | 737 | 1450 |
| Standard Deviation | 6.5 | 23.6 | 48.7 | 13.3 | 41.4 | 104 |
| CV% | 5.7 | 3.2 | 3.3 | 10.7 | 5.6 | 7.2 |
Recovery for Human IL-3 Quantikine ELISA Kit
The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 92 | 78-105 |
| Citrate Plasma (n=0) | 104 | 93-122 |
| EDTA Plasma (n=0) | 108 | 96-117 |
| Heparin Plasma (n=0) | 100 | 93-108 |
| Serum (n=5) | 104 | 96-118 |
Linearity
To assess the linearity of the assay, the following samples spiked with high concentrations of IL-3 were diluted with the appropriate Calibrator Diluent and then assayed.
Scientific Data Images for Human IL-3 Quantikine ELISA Kit
Human IL-3 ELISA Cell Culture Supernate Standard Curve
Human IL-3 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-3
Long Name
Interleukin 3
Alternate Names
IL3, MCGF, MULTI-CSF
Gene Symbol
IL3
Additional IL-3 Products
Product Documents for Human IL-3 Quantikine ELISA Kit
Product Specific Notices for Human IL-3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.