Human IL-34 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # D3400
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Human Milk (13 µL)
Sensitivity
3.06 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)
Product Summary for Human IL-34 Quantikine ELISA Kit
The Quantikine Human IL-34 Immunoassay is a 4.5 hour solid phase ELISA
designed to measure IL-34 in cell culture supernates, cell lysates,
serum, and plasma. It contains NS0-expressed recombinant
human IL-34 and antibodies raised against the recombinant factor. This
immunoassay has been shown to accurately quantitate recombinant human
IL-34. Results obtained using natural human IL-34 showed linear curves
that were parallel to the standard curves obtained using the Quantikine
kit standards. These results indicate that this kit can be used to
determine relative mass values for natural human IL-34.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-34
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 212 | 571 | 1174 | 215 | 597 | 1206 |
| Standard Deviation | 15.5 | 14.4 | 21.2 | 12.8 | 35.4 | 50.0 |
| CV% | 7.3 | 2.5 | 1.8 | 6.0 | 5.9 | 4.1 |
Recovery for Human IL-34 Quantikine ELISA Kit
The recovery of human IL-34 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 97 | 85-104 |
| EDTA Plasma (n=4) | 97 | 90-102 |
| Heparin Plasma (n=4) | 97 | 88-105 |
| Serum (n=4) | 98 | 90-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-34 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. <br>
Scientific Data Images for Human IL-34 Quantikine ELISA Kit
Human IL-34 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-34
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.
IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen . The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.
Long Name
Interleukin 34
Alternate Names
C16orf77, IL34
Gene Symbol
IL34
Additional IL-34 Products
Product Documents for Human IL-34 Quantikine ELISA Kit
Product Specific Notices for Human IL-34 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.