How soon after reconstitution should standards in the Human IL-36 alpha/IL-1F6 DuoSet ELISA Kit, Catalog # DY1078-05, be used, or aliquotted and stored?

Name: Human IL-36 alpha/IL-1F6 DuoSet ELISA
Brand: R&D Systems
SKU: DY1078-05
Rating: 3 (1 reviews)

Standards in this kit are very sensitive and should not be allowed to sit longer than about 15-20 minutes before they are aliquotted and stored, undiluted, at -80C. Standards left at room temperature for longer periods could lead to a significant drop in standard signal on subsequent use. Standards should be used soon after reconstitution or after a new aliquot is thawed.

Human IL-36 alpha/IL-1F6 DuoSet ELISA

R&D Systems, part of Bio-Techne | Catalog # DY1078-05

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

12.5-800 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-36 alpha/IL-1F6 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all IL-36 alpha/IL-1F6 ELISA Kits »

Product Summary for Human IL-36 alpha/IL-1F6 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Interleukin 36 alpha (IL-36α). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human IL-36 alpha/IL-1F6 DuoSet ELISA

Human IL-36 alpha / IL-1F6 ELISA Standard Curve

Kit Contents for Human IL-36 alpha/IL-1F6 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween^® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-36 alpha/IL-1F6

Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Entrez Gene IDs

27179 (Human); 54448 (Mouse)

Gene Symbol

IL36A

Additional IL-36 alpha/IL-1F6 Products

Product Documents for Human IL-36 alpha/IL-1F6 DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human IL-36 alpha/IL-1F6 DuoSet ELISA

For research use only

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