Human IL-6 ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # D6050B
Key Product Details
Assay Length
4.5 hour
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)
Sensitivity
0.626 pg/mL
Assay Range
3.13-300 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human IL-6 Quantikine ELISA Kit
The Quantikine Human IL-6 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure human IL-6 in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 and antibodies raised against the recombinant protein. Natural human IL-6 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human IL-6.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-6.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma- Samples from apparently healthy volunteers were evaluated for the presence
of human IL-6 in this assay. No medical histories were available for the donors used in this
study.
ND=Non-Detectable
Urine - Ten samples from apparently healthy volunteers were evaluated for the presence of human IL-6 in this assay. No medical histories were available for the donors used in this study. All samples measured less than the lowest human IL-6 standard, 3.13 pg/mL.
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Serum (n=30) | 6.90 | 40 | ND-13.9 |
| EDTA plasma (n=30) | 6.82 | 40 | ND-13.4 |
| Heparin plasma (n=30) | 6.89 | 40 | ND-12.9 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106
cells/mL)
were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate. PBMCs were
left untreated or treated with 10 ug/mL PHA for 5 days before collecting conditioned media.
Aliquots of the cell culture supernates were removed and assayed for levels of human IL-6.
| Condition | Day 5 (pg/mL) |
| Unstimulated | 24.0 |
| Stimulated | 10,986 |
Urine - Ten samples from apparently healthy volunteers were evaluated for the presence of human IL-6 in this assay. No medical histories were available for the donors used in this study. All samples measured less than the lowest human IL-6 standard, 3.13 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 38.8 | 94.7 | 205 | 31.8 | 97.4 | 191 |
| Standard Deviation | 0.687 | 2.02 | 4.69 | 1.35 | 3.65 | 8.25 |
| CV% | 1.8 | 2.1 | 2.3 | 4.2 | 3.8 | 4.3 |
Recovery for Human IL-6 Quantikine ELISA Kit
The recovery of human IL-6 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 82-122 |
| EDTA Plasma (n=4) | 97 | 90-102 |
| Heparin Plasma (n=4) | 96 | 90-99 |
| Serum (n=4) | 96 | 89-103 |
| Urine (n=4) | 97 | 89-101 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-6 in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-6 Quantikine ELISA Kit
Human IL-6 Quantikine ELISA Standard Curve (2nd Gen)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filter on the left to drill down to the IL-6 product that best fits your needs.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
Additional IL-6 Products
Product Documents for Human IL-6 Quantikine ELISA Kit
Product Specific Notices for Human IL-6 Quantikine ELISA Kit
For research use only
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