Human IL-7 Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HS750
Key Product Details
Assay Length
18-24 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
0.1 pg/mL
Assay Range
0.156-10 pg/mL (Cell Culture Supernates)
0.25-16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
0.25-16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
Product Summary for Human IL-7 Quantikine HS ELISA Kit
The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.50 | 1.49 | 6.86 | 0.78 | 2.13 | 8.81 |
| Standard Deviation | 0.04 | 0.14 | 0.61 | 0.08 | 0.16 | 0.64 |
| CV% | 8.0 | 9.4 | 8.9 | 10.3 | 7.5 | 7.3 |
Citrate Plasma, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.46 | 5.79 | 11.18 | 0.51 | 6.38 | 12.95 |
| Standard Deviation | 0.03 | 0.19 | 0.44 | 0.06 | 0.50 | 1.30 |
| CV% | 6.5 | 3.3 | 3.9 | 11.8 | 7.8 | 10.0 |
Recovery for Human IL-7 Quantikine HS ELISA Kit
The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 103 | 89-113 |
| Citrate Plasma (n=5) | 100 | 92-110 |
| EDTA Plasma (n=5) | 112 | 83-132 |
| Serum (n=5) | 100 | 91-113 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-7 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-7 Quantikine HS ELISA Kit
Human IL-7 High Sensitivity ELISA Cell Culture Supernate Standard Curve
Human IL-7 High Sensitivity ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-7
Long Name
Interleukin 7
Alternate Names
IL7, Lymphopoietin-1, PBGF
Gene Symbol
IL7
Additional IL-7 Products
Product Documents for Human IL-7 Quantikine HS ELISA Kit
Product Specific Notices for Human IL-7 Quantikine HS ELISA Kit
For research use only
Loading...
Loading...
Loading...