Human Leptin Quantikine QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK398
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
1.03 pg/mL
Assay Range
15.6-1000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Leptin Quantikine QuicKit ELISA
The Quantikine® QuicKit™ Human Leptin Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Leptin levels in cell culture supernates, serum, and plasma. It contains E.
coli-expressed recombinant human Leptin and antibodies raised against the recombinant protein. Results obtained using natural human Leptin showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Leptin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 134 | 709 | 130 | 709 |
| Standard Deviation | 2.84 | 15.8 | 11.9 | 19.7 |
| CV% | 2.1 | 2.2 | 9.2 | 2.8 |
Recovery for Human Leptin Quantikine QuicKit ELISA
The recovery of human Leptin spiked to three levels in samples throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 89 | 80-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Leptin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human Leptin Quantikine QuicKit ELISA
Human Leptin ELISA Standard Curve
Human Leptin QuicKit Spiked Recovery Competitor Comparison
Leptin is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 74% compared to 70% for the top competitor. EDTA plasma recovery is 84% compared to 69% for the top competitor. Heparin plasma recovery is 88% compared to 77% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Human Leptin QuicKit Linearity Competitor Comparison
Samples containing and/or spiked with high concentrations of Leptin in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-106% compared to 71%-122% for the top competitor.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin/OB
Additional Leptin/OB Products
Product Documents for Human Leptin Quantikine QuicKit ELISA
Product Specific Notices for Human Leptin Quantikine QuicKit ELISA
For research use only
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