Human Leptin R Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DOBR00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.128 ng/mL
Assay Range
0.312-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Leptin R Quantikine ELISA Kit
The Quantikine Human Leptin sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Leptin sR in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Leptin R/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Leptin sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Leptin sR.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Leptin sR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.83 | 5.15 | 11.2 | 1.69 | 5.09 | 10.7 |
| Standard Deviation | 0.071 | 0.283 | 0.292 | 0.097 | 0.282 | 0.633 |
| CV% | 3.9 | 5.5 | 2.6 | 5.7 | 5.5 | 5.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 2.34 | 7.01 | 14.6 | 2.34 | 6.95 | 14.3 |
| Standard Deviation | 0.142 | 0.155 | 0.709 | 0.202 | 0.370 | 0.966 |
| CV% | 6.1 | 2.2 | 4.9 | 8.6 | 5.3 | 6.7 |
Recovery for Human Leptin R Quantikine ELISA Kit
The recovery of human Leptin sR spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 91-102 |
| EDTA Plasma (n=4) | 104 | 92-115 |
| Heparin Plasma (n=4) | 103 | 92-113 |
| Serum (n=4) | 106 | 95-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Leptin sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Leptin R Quantikine ELISA Kit
Human Leptin R ELISA Cell Culture Supernate Standard Curve
Human Leptin R ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin R
Leptin receptor (OBR), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OBR, including a long form (OBRL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OBRS) lacking signal-transducing capabilities, have been identified.
Long Name
Leptin Receptor
Alternate Names
B219, CD295, LEPR, LeptinR, OB R
Gene Symbol
LEPR
Additional Leptin R Products
Product Documents for Human Leptin R Quantikine ELISA Kit
Product Specific Notices for Human Leptin R Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.