Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA

R&D Systems, part of Bio-Techne | Catalog # DYC4720-2

R&D Systems, part of Bio-Techne
Discontinued Product
DYC4720-2 has been discontinued. View all WNK1 products.

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

15.6-1000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human, Mouse, Rat

Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

View all WNK1 ELISA Kits »

Product Summary for Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat WNK1 (T60) in cell lysates. An immobilized capture antibody specific for WNK1 binds both phosphorylated and unphosphorylated WNK1 . After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: WNK1

WNK1 belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen ion excretion.

Long Name

WNK Lysine-deficient Protein Kinase 1

Alternate Names

KDP, PHA2C, PRKWNK1

Entrez Gene IDs

65125 (Human); 232341 (Mouse); 116477 (Rat)

Gene Symbol

WNK1

Additional WNK1 Products

Product Documents for Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA

For research use only

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