Human Myeloperoxidase ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DMYE00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Saliva (10 µL), Urine (50 µL)
Sensitivity
0.062 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Urine)
Product Summary for Human Myeloperoxidase Quantikine ELISA Kit
The Quantikine Human MPO Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MPO in cell culture supernates, cell lysates, serum, platelet-poor plasma, saliva, and urine.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human MPO
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human MPO in this assay. No medical histories were available for the donors
used in this study.
Cell Culture Supernates:
HL-60 human acute promyelocytic leukemia cells were cultured in RPMI supplemented with 20% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MPO, and measured 18.2 ng/mL.
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=29) | 100 | 21.4-229 | 65 |
| Platelet-poor EDTA plasma (n=29) | 7.82 | 2.89-27.4 | 4.93 |
| Platelet-poor heparin plasma (n=29) | 9.47 | 3.57-28.4 | 5.27 |
| Saliva (n=10) | 558 | 31.2-1428 | 461 |
| Urine (n=13) | 4.39 | 0.17-13.5 | 4.68 |
Cell Culture Supernates:
Human monocytes were cultured in RPMI supplemented with 10% fetal bovine serum,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were
cultured unstimulated or stimulated with 1 μg/mL LPS for 1 and 6 days. Aliquots of the cell
culture supernates were removed and assayed for human MPO.
| Condition | Day 1 (ng/mL) | Day 6 (ng/mL) |
| Unstimulated | 28.0 | 41.4 |
| Stimulated | 29.9 | 30.3 |
HL-60 human acute promyelocytic leukemia cells were cultured in RPMI supplemented with 20% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MPO, and measured 18.2 ng/mL.
An aliquot of the same HL-60 cells was removed, and the cells were lysed at 5 x 106
cells/mL
in diluted Cell Lysis Buffer 3. The lysate was assayed for human MPO and measured
2673 ng/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.994 | 3.36 | 6.68 | 0.974 | 2.99 | 6.23 |
| Standard Deviation | 0.034 | 0.085 | 0.123 | 0.078 | 0.18 | 0.38 |
| CV% | 3.4 | 2.5 | 1.8 | 8.0 | 6.0 | 6.0 |
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.03 | 3.13 | 6.63 | 1.07 | 3.29 | 6.71 |
| Standard Deviation | 0.024 | 0.046 | 0.172 | 0.12 | 0.26 | 0.55 |
| CV% | 2.3 | 1.5 | 2.6 | 10.8 | 8.0 | 8.2 |
Recovery for Human Myeloperoxidase Quantikine ELISA Kit
The recovery of MPO spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 92-105 |
| Platelet-poor EDTA Plasma (n=4) | 95 | 90-101 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 83-100 |
| Serum (n=4) | 100 | 88-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MPO were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Myeloperoxidase Quantikine ELISA Kit
Human Myeloperoxidase/MPO ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve
Human Myeloperoxidase/MPO ELISA Serum/Plasma/Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Myeloperoxidase/MPO
Additional Myeloperoxidase/MPO Products
Product Documents for Human Myeloperoxidase Quantikine ELISA Kit
Product Specific Notices for Human Myeloperoxidase Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.