Human NT-ProANP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DANP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
0.146 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human NT-ProANP Quantikine ELISA Kit
The Quantikine® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human NT-ProANP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.71 | 6.04 | 11.1 | 1.81 | 5.95 | 11.5 |
| Standard Deviation | 0.069 | 0.224 | 0.583 | 0.129 | 0.426 | 0.842 |
| CV% | 4.0 | 3.7 | 5.3 | 7.1 | 7.2 | 7.3 |
Recovery for Human NT-ProANP Quantikine ELISA Kit
The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 87-114 |
| EDTA Plasma (n=4) | 102 | 84-120 |
| Heparin Plasma (n=4) | 98 | 84-111 |
| Serum (n=4) | 108 | 95-122 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human NT-ProANP Quantikine ELISA Kit
Human NT-ProANP ELISA Standard Curve
Endothelin Induces Expression of Atrial Natriuretic Peptide in iPSC-derived Cardiomyoctyes.
JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (ANP), which is known to be elevated during cardiac hypertrophy, was assessed using the Human NT-ProANP Quantikine® ELISA Kit (Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Atrial Natriuretic Peptide/ANP
Alternate Names
ANF, ANP, Cardionatrin, NPPA, PND
Gene Symbol
NPPA
Additional Atrial Natriuretic Peptide/ANP Products
Product Documents for Human NT-ProANP Quantikine ELISA Kit
Product Specific Notices for Human NT-ProANP Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.