Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPTX30
Discontinued Product
DPTX30 has been discontinued.
An alternative/replacement product is available:
DPTX30B. View all Pentraxin 3/TSG-14 products.
Key Product Details
Assay Length
5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (100 µL), EDTA Plasma (100 µL), Saliva (100 µL)
Sensitivity
0.116 ng/mL
Assay Range
0.31-20 ng/mL (Serum-free Cell Culture Supernates, EDTA Plasma, Saliva)
Product Summary for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PTX3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
EDTA Plasma, Saliva, Serum-free Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 2.61 | 7.72 | 14.1 | 2.75 | 7.94 | 14.5 |
| Standard Deviation | 0.01 | 0.28 | 0.62 | 0.17 | 0.39 | 0.60 |
| CV% | 3.8 | 3.7 | 4.4 | 6.1 | 5.0 | 4.1 |
Recovery for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 83-106 |
| Serum-free Cell Culture Media (n=4) | 101 | 95-108 |
Linearity
To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Human Pentraxin 3/TSG-14 ELISA Standard Curve
Human Pentraxin 3/TSG-14 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Pentraxin 3/TSG-14
Long Name
Tumor Necrosis Factor Stimulated Gene 14
Alternate Names
PTX3, TSG-14, TSG14
Gene Symbol
PTX3
Additional Pentraxin 3/TSG-14 Products
Product Documents for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Product Specific Notices for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.