Human Perforin QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK8011
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
14 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Perforin QuicKit ELISA
The Quantikine QuicKit Human Perforin Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Perforin levels in cell culture supernates, serum, plasma, and cell lysates. It contains recombinant human Perforin and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Perforin showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural Perforin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Perforin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 647 | 2885 | 477 | 2689 |
| Standard Deviation | 36.6 | 77.7 | 33.0 | 95.4 |
| CV% | 5.7 | 2.7 | 6.9 | 3.5 |
Recovery for Human Perforin QuicKit ELISA
The recovery of human Perforin spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 82-132 |
| EDTA Plasma (n=2) | 100 | 81-122 |
| Heparin Plasma (n=2) | 103 | 87-118 |
| Lysis Buffer (n=1) | 118 | 109-127 |
| Serum (n=2) | 97 | 73-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Perforin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human Perforin QuicKit ELISA
Human Perforin Quantikine QuicKit ELISA Standard Curve
Human Perforin QuicKit Serum Recovery
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Perforin
Long Name
Perforin 1 (Pore Forming Protein)
Alternate Names
Cytolysin, FLH2, HPLH2, P1, PFP, PRF1
Gene Symbol
PRF1
Additional Perforin Products
Product Documents for Human Perforin QuicKit ELISA
Product Specific Notices for Human Perforin QuicKit ELISA
For research use only
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