Human PlGF Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HSPG00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.326 pg/mL
Assay Range
3.13-200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human PlGF Quantikine HS ELISA Kit
The Quantikine HS Human PlGF Immunoassay is a 3.5 hour solid phase
ELISA designed to measure PlGF levels in serum and plasma. It contains
E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.
ELISA designed to measure PlGF levels in serum and plasma. It contains
E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PlGF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 27.2 | 57.4 | 108 | 27.3 | 57.7 | 108 |
| Standard Deviation | 0.72 | 1.87 | 2.84 | 1.65 | 3.88 | 7.37 |
| CV% | 2.6 | 3.3 | 2.6 | 6.0 | 6.7 | 6.8 |
Recovery for Human PlGF Quantikine HS ELISA Kit
The recovery of PlGF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 98 | 85-115 |
| Heparin Plasma (n=4) | 90 | 86-97 |
| Serum (n=4) | 92 | 86-97 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of PlGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PlGF Quantikine HS ELISA Kit
Human PlGF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PlGF
Long Name
Placenta Growth Factor
Alternate Names
PGF, PGFL
Gene Symbol
PGF
Additional PlGF Products
Product Documents for Human PlGF Quantikine HS ELISA Kit
Product Specific Notices for Human PlGF Quantikine HS ELISA Kit
For research use only
Loading...
Loading...
Loading...