Human Pro-MMP-1 ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # DMP100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.095 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Human Pro-MMP-1 Quantikine ELISA Kit
The Quantikine Human Pro-MMP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Pro-MMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-1 and antibodies raised against the recombinant factor. The antibodies will not recognize recombinant human active MMP-1 or TIMP bound MMP-1. Natural human Pro-MMP-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human Pro-MMP-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Pro-MMP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.494 | 1.65 | 3.39 | 0.561 | 1.73 | 3.50 |
| Standard Deviation | 0.022 | 0.065 | 0.177 | 0.065 | 0.153 | 0.270 |
| CV% | 4.5 | 4.0 | 5.2 | 11.7 | 8.8 | 7.7 |
Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.702 | 2.13 | 4.43 | 0.738 | 2.34 | 4.66 |
| Standard Deviation | 0.039 | 0.130 | 0.241 | 0.077 | 0.176 | 0.312 |
| CV% | 5.6 | 6.1 | 5.4 | 10.4 | 7.5 | 6.7 |
Recovery for Human Pro-MMP-1 Quantikine ELISA Kit
The recovery of Pro-MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 98-112 |
| Heparin Plasma (n=5) | 92 | 85-102 |
| Serum (n=5) | 97 | 87-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Pro-MMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Scientific Data Images for Human Pro-MMP-1 Quantikine ELISA Kit
Human Pro-MMP-1 ELISA Cell Culture Supernate Standard Curve
Human Pro-MMP-1 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-1
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Long Name
Matrix Metalloproteinase 1
Alternate Names
MMP1
Gene Symbol
MMP1
Additional MMP-1 Products
Product Documents for Human Pro-MMP-1 Quantikine ELISA Kit
Product Specific Notices for Human Pro-MMP-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.