Human Pro-MMP-13 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DM1300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL)
Sensitivity
21.3 pg/mL
Assay Range
78-5000 pg/mL (Cell Culture Supernates)
Product Summary for Human Pro-MMP-13 Quantikine ELISA Kit
The Quantikine Human Pro-MMP-13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure pro-MMP-13 in cell culture supernates. It contains NS0-expressed recombinant human pro-MMP-13 and antibodies raised against the recombinant factor. The Quantikine kit will not detect recombinant human active MMP-13 existing in either free or TIMP-bound form. Natural human pro-MMP-13 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human pro-MMP-13.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human pro-MMP-13
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 485 | 1462 | 3521 | 533 | 1561 | 3823 |
| Standard Deviation | 18.2 | 64.0 | 93.0 | 27.9 | 56.1 | 143 |
| CV% | 3.8 | 4.4 | 2.6 | 5.2 | 3.6 | 3.7 |
Recovery for Human Pro-MMP-13 Quantikine ELISA Kit
The recovery of pro-MMP-13 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 101 | 86-112 |
| Cell Culture Supernates (n=3) | 98 | 85-111 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of pro-MMP-13 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Pro-MMP-13 Quantikine ELISA Kit
Human Pro-MMP-13 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-13
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Long Name
Matrix Metalloproteinase 13
Alternate Names
MMP13
Entrez Gene IDs
4322 (Human)
Gene Symbol
MMP13
Additional MMP-13 Products
Product Documents for Human Pro-MMP-13 Quantikine ELISA Kit
Product Specific Notices for Human Pro-MMP-13 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.