Human SCF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCK00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity
9 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human SCF Quantikine ELISA Kit
The Quantikine Human SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure SCF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human soluble SCF (KL-1 variant) and antibodies raised against this recombinant factor. Results obtained for naturally occurring human SCF samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SCF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human SCF. This assay cross-reacts 15.6% with Canine SCF, 16.1% with Feline SCF, 3.5% with Mouse SCF and 1.0% with Rat SCF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 153 | 709 | 1427 | 151 | 729 | 1552 |
| Standard Deviation | 3.6 | 11.6 | 61.0 | 12.1 | 45.8 | 125 |
| CV% | 2.4 | 1.6 | 4.3 | 8.0 | 6.3 | 8.1 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 139 | 655 | 1419 | 142 | 703 | 1574 |
| Standard Deviation | 5.5 | 12.9 | 54.0 | 14.6 | 36.0 | 94.0 |
| CV% | 4.0 | 2.0 | 3.8 | 10.3 | 5.1 | 6.0 |
Recovery for Human SCF Quantikine ELISA Kit
The recovery of SCF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 85 | 75-93 |
| Citrate Plasma (n=8) | 94 | 84-112 |
| EDTA Plasma (n=8) | 98 | 82-110 |
| Heparin Plasma (n=8) | 99 | 88-116 |
| Serum (n=8) | 99 | 87-111 |
Linearity
To assess the linearity of the assay, the following biological samples were diluted with the appropriate Calibrator Diluent and then assayed. The cell culture media was spiked with exogenous SCF.
Scientific Data Images for Human SCF Quantikine ELISA Kit
Human SCF/c-kit Ligand ELISA Cell Culture Supernate Standard Curve
Human SCF/c-kit Ligand ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SCF/c-kit Ligand
Long Name
Stem Cell Factor
Alternate Names
c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF
Gene Symbol
KITLG
Additional SCF/c-kit Ligand Products
Product Documents for Human SCF Quantikine ELISA Kit
Product Specific Notices for Human SCF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.