Human TLR2 Quantikine ELISA Kit

R&D Systems, part of Bio-Techne | Catalog # DTLR20

R&D Systems, part of Bio-Techne
Discontinued Product
DTLR20 has been discontinued. View all TLR2 products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Cell Lysates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (34 µL), Urine (100 µL), Human Milk (10 µL)

Sensitivity

0.109 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

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Product Summary for Human TLR2 Quantikine ELISA Kit

The Quantikine Human TLR2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TLR2 in cell culture supernates, cell lysates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human TLR2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TLR2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TLR2.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human TLR2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, Human Milk, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.32 2.72 5.47 1.40 2.82 5.73
Standard Deviation 0.032 0.055 0.109 0.105 0.225 0.427
CV% 2.4 2.0 2.0 7.5 8.0 7.5

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.43 3.01 5.82 1.48 3.12 6.20
Standard Deviation 0.051 0.065 0.155 0.128 0.310 0.460
CV% 3.6 2.2 2.7 8.6 9.9 7.4

Recovery for Human TLR2 Quantikine ELISA Kit

The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 92-98
EDTA Plasma (n=4) 98 86-107
Heparin Plasma (n=4) 98 88-105
Serum (n=4) 99 84-111
Urine (n=4) 98 91-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TLR2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human TLR2 ELISA Linearity

Scientific Data Images for Human TLR2 Quantikine ELISA Kit

Human TLR2 ELISA Serum/Plasma Standard Curve

Human TLR2 ELISA Serum/Plasma Standard Curve

Human TLR2 ELISA Cell Culture Supernate/Cell Lysate/ Saliva/Urine/Human Milk Standard Curve

Human TLR2 ELISA Cell Culture Supernate/Cell Lysate/ Saliva/Urine/Human Milk Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TLR2

TLRs make up a family of pattern recognition receptors that play important roles in the innate immune response. Broad classes of pathogens (e.g. viruses, bacteria, and fungi) constitutively express a set of mutation-resistant molecules called pathogen-associated molecular patterns (PAMPs). These microbial molecular markers may be composed of proteins, carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades that promote the immune response. Structurally, TLRs are type I transmembrane receptors that possess varying numbers of extracellular N-terminal leucine-rich repeat (LRR) motifs, followed by a cysteine-rich region, a TM domain, and an intracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the larger IL-1 R/TLR superfamily.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Entrez Gene IDs

7097 (Human); 24088 (Mouse); 310553 (Rat)

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for Human TLR2 Quantikine ELISA Kit

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TLR2 Quantikine ELISA Kit

For research use only

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