Human TNF-alpha QuantiGlo ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # QTA00C
Key Product Details
Assay Length
5.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), Plasma (100 µL)
Sensitivity
0.481 pg/mL
Assay Range
2.24-7000 pg/mL
Product Summary for Human TNF-alpha QuantiGlo ELISA Kit
The QuantiGlo Human TNF-alpha Immunoassay is a 5 hour solid phase chemiluminescent ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E.
coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha. Results obtained using naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the QuantiGlo® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Chemiluminescent - Luminometer (Luminol)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human TNF-alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Recovery for Human TNF-alpha QuantiGlo ELISA Kit
The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 104 | 98-100 |
| EDTA Plasma (n=4) | 96 | 90-104 |
| Heparin Plasma (n=4) | 95 | 88-100 |
| Serum (n=4) | 91 | 87-97 |
Linearity
<div>To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TNF-alpha in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.</div>
Scientific Data Images for Human TNF-alpha QuantiGlo ELISA Kit
HIV-1 Gag p24 Standard Curve
Human TNF-alpha Chemiluminescent ELISA Calibrator Diluent RD5P (1X) Standard Curve
Human IL-2 ELISA Cell Culture Supernate Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF-alpha
Human TNF-alpha is synthesized as a 26 kDa type II transmembrane
protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane
segment, and a 177 aa extracellular domain (ECD) (12, 13). Within the ECD,
human TNF-alpha shares 97% aa sequence identity with rhesus monkey, and 71%-92% aa
identity with bovine, canine, cotton rat, equine, feline, mouse, porcine, and
rat TNF-alpha. It is produced by a wide variety of immune, epithelial, endothelial,
and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently
linked homotrimer which is expressed on the cell surface (14). Cell surface
TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and
generate its own downstream cell signaling following ligation by soluble TNF RI
(15, 16). Shedding of membrane bound TNF-alpha by TACE/ ADAM17 releases the
bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain
(17-19).
TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (20, 21) and the
hematopoietic cell-restricted 78-80 kDa TNF RII (22, 23), both of which are
also expressed as homotrimers (1, 24). Both type I and type II receptors bind
TNF-alpha with comparable affinity and can promote NF kappaB activation (25-28). Only
TNF RI, however, contains a cytoplasmic death domain which triggers the
activation of apoptosis (3, 29). Soluble forms of both types of receptors are
released into human serum and urine and can neutralize the biological activity
of TNF-alpha (30-32).
Long Name
Tumor Necrosis Factor alpha
Alternate Names
Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2
Entrez Gene IDs
Gene Symbol
TNF
Additional TNF-alpha Products
Product Documents for Human TNF-alpha QuantiGlo ELISA Kit
Product Specific Notices for Human TNF-alpha QuantiGlo ELISA Kit
For research use only
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