Human Total ER alpha/NR3A1 DuoSet IC ELISA
R&D Systems, part of Bio-Techne | Catalog # DYC5715-2
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
15.6-1000 pg/mL
Sample Type
Cell Lysates
Reactivity
Human
Human Total ER alpha/NR3A1 DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human Total ER alpha/NR3A1 DuoSet IC ELISA
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total ER alpha / NR3A1 in cell lysates. An immobilized capture antibody specific for ER alpha / NR3A1 binds both phosphorylated and unphosphorylated ER alpha / NR3A1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Total ER alpha/NR3A1 DuoSet IC ELISA
Standard Curve
Kit Contents for Human Total ER alpha/NR3A1 DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ER alpha/NR3A1
Estrogen receptor alpha (ER alpha, NR3A1) is a member of the steroid receptor family. The natural ligand for ER is the classic estrogenic compound 17 beta-estradiol. ER alpha is expressed in a wide variety of tissues such as bones, uterus, bladder, ovary, prostate, testis, epididymis, kidney, breast, heart, vessel wall, pituitary and hypothalamus. The estrogen receptor has a variety of central physiological roles, including those involved in maintenance of the reproductive, cardiovascular, musculoskeletal and central nervous systems. ER alpha has been shown to form homodimers as well as heterodimers with ER beta. Both ER alpha and ER beta can give rise to numerous isoforms.
Long Name
Estrogen Receptor alpha
Alternate Names
ESR1, NR3A1
Entrez Gene IDs
2099 (Human)
Gene Symbol
ESR1
Additional ER alpha/NR3A1 Products
Product Documents for Human Total ER alpha/NR3A1 DuoSet IC ELISA
Product Specific Notices for Human Total ER alpha/NR3A1 DuoSet IC ELISA
For research use only
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