Human Total MMP-8 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMP800B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (25 µL), Platelet-poor EDTA Plasma (50 µL), Heparin Plasma (25 µL), Platelet-poor Heparin Plasma (50 µL), Saliva (10 µL)
Sensitivity
0.058 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva)
Product Summary for Human Total MMP-8 Quantikine ELISA Kit
The Quantikine® Human Total MMP-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total MMP-8 (pro- and active MMP-8) in cell culture supernates, tissue lysates, serum, plasma, platelet-poor plasma, and saliva. It contains NS0-expressed recombinant human MMP-8, and antibodies raised against the recombinant protein. Natural human MMP-8 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MMP-8.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MMP-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.764 | 2.38 | 4.55 | 0.836 | 2.41 | 5.07 |
| Standard Deviation | 0.049 | 0.070 | 0.299 | 0.055 | 0.171 | 0.270 |
| CV% | 6.4 | 2.9 | 6.6 | 6.6 | 7.1 | 5.3 |
Recovery for Human Total MMP-8 Quantikine ELISA Kit
The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 93-103 |
| EDTA Plasma (n=4) | 92 | 86-99 |
| Extraction Buffer (1X) (n=2) | 84 | 83-85 |
| Heparin Plasma (n=4) | 92 | 86-99 |
| Platelet-poor EDTA Plasma (n=4) | 85 | 79-91 |
| Platelet-poor Heparin Plasma (n=4) | 84 | 78-89 |
| Saliva (n=4) | 96 | 86-111 |
| Serum (n=4) | 101 | 93-109 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total MMP-8 in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Total MMP-8 Quantikine ELISA Kit
Human Total MMP-8 Standard Curve
Human Total MMP-8 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-8
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Long Name
Matrix Metalloproteinase 8
Alternate Names
Collagenase 2, MMP8
Gene Symbol
MMP8
Additional MMP-8 Products
Product Documents for Human Total MMP-8 Quantikine ELISA Kit
Product Specific Notices for Human Total MMP-8 Quantikine ELISA Kit
For research use only
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