Human TREM-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTRM10C
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL)
Sensitivity
15.2 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human TREM-1 Quantikine ELISA Kit
The Quantikine Human TREM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TREM-1 levels in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TREM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human TREM-1 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TREM-1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human TREM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 479 | 1226 | 2217 | 511 | 1286 | 2254 |
| Standard Deviation | 16.8 | 41.5 | 72.1 | 25.0 | 57.7 | 90.7 |
| CV% | 3.5 | 3.4 | 3.3 | 4.9 | 4.5 | 4.0 |
Recovery for Human TREM-1 Quantikine ELISA Kit
The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 94-112 |
| Cell Lysis Buffer (n=4) | 88 | 78-96 |
| EDTA Plasma (n=4) | 99 | 92-105 |
| Heparin Plasma (n=4) | 96 | 87-104 |
| Serum (n=4) | 97 | 84-107 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human TREM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TREM-1 Quantikine ELISA Kit
Human TREM-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TREM-1
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Alternate Names
CD354, TREM1
Gene Symbol
TREM1
Additional TREM-1 Products
Product Documents for Human TREM-1 Quantikine ELISA Kit
Product Specific Notices for Human TREM-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.