Human u-Plasminogen Activator/Urokinase Quantikine ELISA
R&D Systems, part of Bio-Techne | Catalog # DUPA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (17 µL), EDTA Plasma (17 µL), Heparin Plasma (17 µL), Urine (10 µL)
Sensitivity
4.17 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human u-Plasminogen Activator/Urokinase Quantikine ELISA
The Quantikine Human u-Plasminogen Activator/Urokinase Immunoassay is a 4.5 hour solid phase ELISA designed to measure uPA in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human uPA. Results obtained using natural human uPA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human uPA.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human uPA. This assay also recognizes uPA complexed with PAI-1 or uPAR.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 230 | 629 | 1219 | 214 | 614 | 1188 |
| Standard Deviation | 4.8 | 8.9 | 29.3 | 15.1 | 39.8 | 82.0 |
| CV% | 2.1 | 1.4 | 2.4 | 7.1 | 6.5 | 6.9 |
Recovery for Human u-Plasminogen Activator/Urokinase Quantikine ELISA
The recovery of uPA spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 102 | 93-115 |
| Cell Lysates (n=4) | 97 | 81-116 |
| EDTA Plasma (n=4) | 87 | 83-94 |
| Heparin Plasma (n=4) | 89 | 81-110 |
| Serum (n=4) | 87 | 81-93 |
| Urine (n=4) | 98 | 81-119 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of uPA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human u-Plasminogen Activator/Urokinase Quantikine ELISA
Human u-Plasminogen Activator (uPA)/Urokinase ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: u-Plasminogen Activator (uPA)/Urokinase
Long Name
Urokinase-type Plasminogen Activator
Alternate Names
PLAU, uPA, uPlasminogen Activator, Urokinase
Gene Symbol
PLAU
Additional u-Plasminogen Activator (uPA)/Urokinase Products
- All Products for u-Plasminogen Activator (uPA)/Urokinase
- u-Plasminogen Activator (uPA)/Urokinase Activity Assays
- u-Plasminogen Activator (uPA)/Urokinase cDNA Clones
- u-Plasminogen Activator (uPA)/Urokinase ELISA Kits
- u-Plasminogen Activator (uPA)/Urokinase Lysates
- u-Plasminogen Activator (uPA)/Urokinase Primary Antibodies
- u-Plasminogen Activator (uPA)/Urokinase Proteins and Enzymes
- u-Plasminogen Activator (uPA)/Urokinase Simple Plex
Product Documents for Human u-Plasminogen Activator/Urokinase Quantikine ELISA
Product Specific Notices for Human u-Plasminogen Activator/Urokinase Quantikine ELISA
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.