Human VCAM-1/CD106 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DVC00
Key Product Details
Assay Length
2 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
1.26 ng/mL
Assay Range
6.25-200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human VCAM-1/CD106 Quantikine ELISA Kit
The Quantikine Human sVCAM-1 Immunoassay is a 2 hour solid-phase ELISA designed to measure human sVCAM-1 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sVCAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sVCAM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human sVCAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 583 | 1423 | 2421 | 670 | 1571 | 2726 |
| Standard Deviation | 13.6 | 49.5 | 86.4 | 52.1 | 120.9 | 151.2 |
| CV% | 2.3 | 3.5 | 3.6 | 7.8 | 7.7 | 5.5 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of sVCAM-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human VCAM-1/CD106 Quantikine ELISA Kit
Human VCAM-1/CD106 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VCAM-1/CD106
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
Additional VCAM-1/CD106 Products
Product Documents for Human VCAM-1/CD106 Quantikine ELISA Kit
Product Specific Notices for Human VCAM-1/CD106 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.