Human VE-Cadherin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCADV0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.236 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human VE-Cadherin Quantikine ELISA Kit
The Quantikine Human VE-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VE-Cadherin in cell culture supernates, serum, and plasma. It contains NS0-derived recombinant human VE-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VE-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VE-Cadherin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human VE-Cadherin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 13.4 | 34.6 | 65.4 | 12.8 | 31.8 | 64.5 |
| Standard Deviation | 0.68 | 1.63 | 2.05 | 0.92 | 1.64 | 2.86 |
| CV% | 5.1 | 4.7 | 3.1 | 7.2 | 5.1 | 4.4 |
Recovery for Human VE-Cadherin Quantikine ELISA Kit
The recovery of VE-Cadherin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 94-108 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of VE-Cadherin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human VE-Cadherin Quantikine ELISA Kit
Human VE-Cadherin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VE-Cadherin
The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.
Long Name
Vascular Endothelium Cadherin
Alternate Names
Cadherin-5, CD144, CDH5, VECadherin
Gene Symbol
CDH5
Additional VE-Cadherin Products
Product Documents for Human VE-Cadherin Quantikine ELISA Kit
Product Specific Notices for Human VE-Cadherin Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.