Human VEGF-C Quantikine ELISA Kit

Name: Human VEGF-C Quantikine ELISA Kit
Brand: R&D Systems
SKU: DVEC00
Rating: 4 (3 reviews)

R&D Systems, part of Bio-Techne | Catalog # DVEC00

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (50 µL), Platelet-poor Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL)

Sensitivity

48.4 pg/mL

Assay Range

109-7000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma, Saliva)

View all VEGF-C ELISA Kits »

Product Summary for Human VEGF-C Quantikine ELISA Kit

The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	572	1622	3243	520	1527	3069
Standard Deviation	39.3	54.4	136	50.1	102	200
CV%	6.9	3.4	4.2	9.6	6.7	6.5

EDTA Plasma, Heparin Plasma, Platelet-poor Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	512	1543	3195	514	1540	3066
Standard Deviation	33.8	54.2	136	43.6	110	198
CV%	6.6	3.5	4.3	8.5	7.2	6.4

Recovery for Human VEGF-C Quantikine ELISA Kit

The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	107	93-115
EDTA Plasma (n=4)	101	96-110
Heparin Plasma (n=4)	97	88-103
Serum (n=4)	99	89-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VEGF-C were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human VEGF-C Quantikine ELISA Kit

Human VEGF-C ELISA Cell Culture Supernate/Saliva Standard Curve

Human VEGF-C ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Entrez Gene IDs

7424 (Human); 22341 (Mouse)

Gene Symbol