Human Vitamin D BP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DVDBP0B
Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
0.338 ng/mL
Assay Range
6.25-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human Vitamin D BP Quantikine ELISA Kit
The Quantikine® Human Vitamin D BP immunoassay is a 3.5 hour sandwich-type solid phase ELISA designed to measure human Vitamin D BP in cell culture supernates, serum, plasma, and urine. It contains HEK293-expressed recombinant human Vitamin D BP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Vitamin D BP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Vitamin D BP.
Read more about the Vitamin D BP specificity in this White Paper.
Read more about the Vitamin D BP specificity in this White Paper.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Vitamin D BP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 10.9 | 31.7 | 63.7 | 10.1 | 30.8 | 61.9 |
| Standard Deviation | 0.244 | 0.423 | 1.36 | 0.661 | 0.664 | 1.31 |
| CV% | 2.2 | 1.3 | 2.1 | 6.5 | 2.2 | 2.1 |
Recovery for Human Vitamin D BP Quantikine ELISA Kit
The recovery of human Vitamin D BP spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 93-117 |
| Urine (n=4) | 89 | 78-96 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Vitamin D BP were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Vitamin D BP Quantikine ELISA Kit
Human Vitamin D BP ELISA Standard Curve
Human Vitamin D BP Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Vitamin D BP
Long Name
Vitamin D Binding Protein
Alternate Names
DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP
Gene Symbol
GC
Additional Vitamin D BP Products
Product Documents for Human Vitamin D BP Quantikine ELISA Kit
Product Specific Notices for Human Vitamin D BP Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.