Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91178

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (25 uL)

Sensitivity

1.5 pg/mL

Assay Range

4.7 - 300 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into human serum and cell culture supernatant. There was no crossreactivity detected, notably not with IFN alpha and IFN gamma.

Description

The human IFN-omega ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-omega.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.90%

Inter-Assay Precision (Precision between assays) CV% < 6.80%

Recovery for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 76% to 120% with an overall mean recovery of 91%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

Human IFN-omega/IFNW1 ELISA Kit [NBP1-91178] - Data for Human IFN-omega Platinum ELISA

Kit Contents for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

  • 1 vial (1.1 ml) monoclonal Coating Antibody to human IFN-omega (100 ug/ml)
  • 1 vial (11 ul) Streptavidin-HRP
  • 1 vial (30 ul) human IFN-omega Standard protein, 60 ng/ml
  • 1 vial (55 ul) Biotin-Conjugate anti-human IFN-omega monoclonal antibody
  • 2 vials (50 ml) Sample Diluent

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IFN-omega

In the human genome, the IFN-omega gene family consists of seven members located on chromosome 9. However, only one of these genes is functional giving rise to the IFN-omega protein, whereas the others are non-functional pseudogenes. The IFN-omega gene is not expressed in unstimulated cells. Viral infection results in expression of the gene giving rise to the N-glycosylated protein consisting of 172 or 174 amino acids and an apparent molecular mass of about 25kDa. A single carbohydrate group consists mainly of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid. In quantitative terms, IFN-omega is a major component of human leukocyte IFN; with a contribution to its total antiviral activity estimated to be in the range of 10 -15 %.

Long Name

Interferon omega

Alternate Names

IFNomega, IFNW1

Gene Symbol

IFNW1

Additional IFN-omega Products

Product Documents for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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