Human IgA ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60057

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.24 ng/mL

Assay Range

0.24 ng/mL - 1000 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human IgA. No significant cross-reactivity or interference between human IgA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IgA and all the analogues, therefore, cross reaction may still exist.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 8

Scientific Data Images for Human IgA ELISA Kit (Colorimetric)

ELISA: Human IgA ELISA Kit (Colorimetric) [NBP2-60057]
Human IgA ELISA Kit (Colorimetric)

Human IgA ELISA Kit (Colorimetric) [NBP2-60057] -

Human IgA ELISA Kit (Colorimetric) [NBP2-60057] - Standard Curve Reference

Kit Contents for Human IgA ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgA

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IgA is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Immunoglobulin A

Alternate Names

Immunoglobulin A

Gene Symbol

IGHA1

Additional IgA Products

Product Documents for Human IgA ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IgA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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