Human IgE ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12187

Novus Biologicals, part of Bio-Techne
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NBP3-12187
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cerebrospinal Fluid (CSF), Milk, Urine

Assay Range

0.69 - 500 ng / mL

View all IgE ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Detection method: Biotinylated-Streptavidin-HRP

Scientific Data Images for Human IgE ELISA Kit (Colorimetric)

ELISA: Human IgE ELISA Kit (Colorimetric) [NBP3-12187]

ELISA: Human IgE ELISA Kit (Colorimetric) [NBP3-12187]

ELISA: Human IgE ELISA Kit [NBP3-12187] - Typical standard curve.

Kit Contents for Human IgE ELISA Kit (Colorimetric)

  • Dilution Buffer C (20X) - 25 mL
  • HRP Solution A - 12 mL
  • Human Albumin Detection Antibody - 12 mL
  • Human Albumin Pre-Coated 96-well Strip Plate - 1 EA
  • Human Albumin Standard (500 ng / vial) - 2 EA
  • Sealing Tape - 6 sheets
  • Stop Solution - 12 mL
  • TMB Substrate - 12 mL
  • Wash Buffer (20X) - 50 mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: IgE

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Gene Symbol

IGHE

Additional IgE Products

Product Documents for Human IgE ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IgE ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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