Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60060

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates and tissue homogenates (50 - 100 uL)

Sensitivity

0.151 ng/mL

Assay Range

0.156 ng/mL - 10 ng/mL

View all IGF-I/IGF-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This assay has high sensitivity and excellent specificity for detection of rat IGF-1. No significant cross-reactivity or interference between rat IGF-1 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

ELISA: Rat IGF-I/IGF-1 ELISA Kit (Colorimetric) [NBP2-60060]

ELISA: Rat IGF-I/IGF-1 ELISA Kit (Colorimetric) [NBP2-60060]

ELISA: Rat IGF-I ELISA Kit (Colorimetric) [NBP2-60060] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

Rat IGF-I/IGF-1 ELISA Kit (Colorimetric) [NBP2-60060] -

Rat IGF-I/IGF-1 ELISA Kit (Colorimetric) [NBP2-60060] - Standard Curve Reference

Kit Contents for Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IGF-I/IGF-1

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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