Human IGFBP-1 ELISA Kit (Colorimetric)

Name: Human IGFBP-1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-69809

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69809

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human IGFBP-1 in samples. No significant cross-reactivity or interference between Human IGFBP-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.04%

Inter-Assay Precision (Precision between assays) CV% < 6.81%

Recovery for Human IGFBP-1 ELISA Kit (Colorimetric)

Recovery

91-110%

Linearity

Scientific Data Images for Human IGFBP-1 ELISA Kit (Colorimetric)

ELISA: Human IGFBP-1 ELISA Kit (Colorimetric) [NBP2-69809] -

ELISA: Human IGFBP-1 ELISA Kit (Colorimetric) [NBP2-69809] - Standard Curve Reference

Kit Contents for Human IGFBP-1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Reference Standard & Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IGFBP-1

IGFBP1 is a gene that codes for an IGF-binding protein that circulates in the plasma and prolongs the half-life of IGFs and works to either inhibit of stimulate the growth promoting effects while also promoting cell migration and has a length of 259 amino acids and a weight of approximately 28 kDa. Current studies are being done on several diseases and disorders relating to this gene including protein-energy malnutrition, pre-eclampsia, persistent fetal circulation syndrome, Silver-Russell syndrome, growth hormone deficiency, Ehlers-Danlos syndrome, glucose intolerance, Prader-Willi syndrome, and chronic fatigue syndrome. IGFBP1 has also been shown to have interactions with IGF1, IGF2, MMP26, TF, and ARNT in pathways such as the myometrial relaxation and contraction, HIF-1-alpha transcription factor, IGF regulation, and protein metabolism pathways.

Long Name

Insulin-like Growth Factor Binding Protein 1

Alternate Names

IGFBP1

Gene Symbol