Rat IgG ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1952

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Biological Samples, Plasma, Serum (100 uL)

Sensitivity

2.894

Assay Range

25 to 800 ng/mL

View all IgG ELISA Kits »

Product Specifications

Assay Type

Two-site enzyme-linked immunoassay

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Description

IgG (Rat) ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay for the quantitative determination of IgG in rat biological samples.

Kit Contents for Rat IgG ELISA Kit (Colorimetric)

  • Anti-Rat IgG ELISA Micro Plate: Twelve removable eight (8) well strips in well holder frame. Each well is coated with affinity purified anti-Rat IgG.
  • Chromogen-Substrate Solution: One vial containing 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  • Diluent Concentrate (Running buffer): One bottle containing 5X concentrated diluent running buffer
  • Enzyme-Antibody Conjugate 100X: One vial containing affinity purified anti- Rat IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  • Rat IgG Calibrator: One vial containing a lyophilized Rat IgG calibrator.
  • Stop Solution: One vial containing 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
  • Wash Solution Concentrate: One bottle containing 20X concentrated wash solution.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rat IgG ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IgG ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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