Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91187

Novus Biologicals, part of Bio-Techne
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NBP1-91187
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine (50 uL)

Sensitivity

1.1 pg/mL

Assay Range

1.6 - 100 pg/mL

View all IL-1 alpha/IL-1F1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-1 alpha positive serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.40%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

Recovery

The amount of endogenous human IL-1 alpha in unspiked samples was subtracted from the spike values. The overall mean recovery was 92%.

Scientific Data Images for Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

Human IL-1 alpha/IL-1F1 ELISA Kit [NBP1-91187] - Data for Human IL-1alpha Platinum ELISA

Kit Contents for Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-1alpha
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-1alpha polyclonal antibody
  • Blue-Dye
  • Green-Dye
  • Human IL-1alpha Standard lyophilized, 200 pg/mL upon reconstitution
  • Red-Dye
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-1 alpha/IL-1F1

The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct human IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation. IL-1alpha is an extracellular peptide of 17 kDa, its activity has been demonstrated in various biological fluids including serum, synovial fluid, gingival fluid, amniotic fluid, sputum, cerebrosinal fluid, urine, and bronchoalveolar lavage (BAL) fluid. Elevated serum or blood levels of IL-1alpha have been found in patients with total hip replacement/ arthroplasties, in patients with recently diagnosted IDDM (Insulin-Dependent Diabetes Mellitus), in case of several carcinomas such as head and neck cancer, pancreatic cancer and thyroid cancer, in experimental acute pyelonephritis, in acute viral hepatitis and in septic shock.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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