Does this kit allow for detection of IL-13 bound to a soluble receptor?

Name: Human IL-13 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-91176

Our human IL-13 ELISA kit with catalogue number NBP1-91176 is intended for the quantitative detection of human IL-13, and suitable sample types include cell culture supernatant, serum, plasma and amniotic fluid. An anti-human IL-13 coating antibody is adsorbed onto microwells, and any human IL-13 present in the sample binds to this antibody; biotin-conjugated anti-human IL-13 antibody and streptavidin-HRP are then used for detection. A purified human IL-13 standard (provided with the kit) is used as a control. Unfortunately we have no specific testing data to confirm whether the antibodies in this kit would be able to detect IL-13 once it has become bound to a soluble receptor. Since we have not epitope-mapped the antibodies that are used in the kit, we do not know exactly where on the IL-13 protein they bind, and if the receptor was to block these binding sites then the kit would not work optimally.

Human IL-13 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91176

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) (50 uL)

Sensitivity

0.7 pg/mL

Assay Range

1.6 - 100 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human IL-13. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-13 positive serum. There was no crossreactivity detected.

Description

The human IL-13 ELISA is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.00%

Inter-Assay Precision (Precision between assays) CV% < 4.60%

Recovery for Human IL-13 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 93% to 110% with an overall mean recovery of 101%

Scientific Data Images for Human IL-13 ELISA Kit (Colorimetric)

Human IL-13 ELISA Kit [NBP1-91176] - Data for Human IL-13 Platinum ELISA

Kit Contents for Human IL-13 ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-13
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-human IL-13 monoclonal antibody
Human IL-13 Standard lyophilized, 200 pg/mL upon reconstitution
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-13

Human IL-13 is a nonglycosylated protein of 132 amino acids with a molecular weight of 12 kDa. The IL-13 gene is located on chromosome 5q23-31, in the same region as the genes encoding IL-3, IL-4, IL-5 and GM-CSF. It is produced by activated CD4+ and CD8+ T cells, but the expression seems to be more abundant in CD4+ T cells. Although it is a Th2 cytokine in the mouse, it appears to be produced by Th0, Th1, and Th2-like human T cell clones. IL-13 induces CD23 expression on B cells, promotes B cell proliferation in combination with anti-Ig or CD40 antibodies, and stimulates secretion IgE, and IgG4. IL-13 has also been shown to prolong survival of human monocytes and increases surface expression of MHC class II, CD23, and members of the integrin superfamily, like CD11b, CD11c, CD18, CD29 and CD49e. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. The capacity of IL-13 to induce IgE synthesis indicates that IL-13 may play an important regulatory role in IgE-mediated atopic diseases. The measurement of IL-13 in body fluids may thus provide further information about the pathophysiology of atopic diseases. Furthermore, IL-13 inhibits HIV-1 replication in primary culture-derived macrophages and represents a candidate cytokine for the suppression of HIV infection within monocytes and macrophages in vivo.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Gene Symbol

IL13

Additional IL-13 Products

Product Documents for Human IL-13 ELISA Kit (Colorimetric)

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Protocols & Manuals

Download Manual - Human IL-13 ELISA Kit (Colorimetric)

COA

SDS

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Product Specific Notices for Human IL-13 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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