Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92705

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) (50 uL)

Sensitivity

1.0 pg/mL

Assay Range

1.6 - 100 pg/mL

View all IL-17/IL-17A ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

Crossreactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-17A positive sample. There was no crossreactivity detected, notably not with rat IFN gamma, rat TNF alpha, rat IL-1 alpha, rat IL-4, rat MCP-1, rat GM-CSF.

Description

The rat IL-17A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-17A.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.5%

Inter-Assay Precision (Precision between assays) CV% < 7.6%

Recovery for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Recovery

Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

ELISA: Rat IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-92705]

ELISA: Rat IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-92705]

ELISA: IL-17/IL-17A ELISA Kit [HRP] [NBP1-92705] - Standard curve

Kit Contents for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-17A
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-rat IL-17A monoclonal antibody
  • Rat IL-17A Standard lyophilized, 200 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17/IL-17A

IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction, as well as increased survival locally. IL-17A and IL-17F production by T lymphocytes is regulated by IL-23 independent of T cell receptor activation. The Th1 and Th2 cell classification has until recently provided the framework for understanding CD4+ T cell biology and the interplay between innate and adaptive immunity. The Th17 lineage produces interleukin 17, which is highly proinflammatory and induces severe autoimmunity. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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