Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76580

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all IL-18 BPa ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat IL18BP in samples. No significant cross-reactivity or interference between Rat IL18BP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.65%

Inter-Assay Precision (Precision between assays) CV% < 4.96%

Recovery for Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

Recovery

84-100%

Linearity

Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

Scientific Data Images for Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

ELISA: Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric) [NBP2-76580] -

ELISA: Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric) [NBP2-76580] - Standard Curve Reference

Kit Contents for Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-18 BPa

IL-18BP which binds to IL-18, also known as interferon-gamma inducing factor (IGIF) which is a pro-inflammatory cytokine that belongs to the IL-1 family (1). IL-18 is a pleiotropic factor involved in the regulation of both innate and acquired immune responses, playing a key role in autoimmune, inflammatory, and infectious diseases (2). Interleukin-18 binding protein (IL18-BP) is an inhibitor of the pro-inflammatory cytokine IL18. The recombinant forms of IL18BP did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity (3). IL-18 plays an important role in host defense against microbial pathogens. Many poxviruses encode homologous IL-18BP that neutralizes IL-18 activity. Data show that IL-18BP prevents IL-18 from binding to IL-18R by interacting with three residues that are part of the binding site for IL-18Ralpha (4).

Long Name

Interleukin 18 Binding Protein Isoform a

Alternate Names

IL18 BPa, IL18BP

Gene Symbol

IL18BP

Additional IL-18 BPa Products

Product Documents for Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-18 BPa/IL18BP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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