Rat IL-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92704

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

11.0 pg/mL

Assay Range

62.5 - 2,000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-2 positive serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Rat IL-2 ELISA Kit (Colorimetric)

Recovery

The unspiked serum was used as blank in these experiments. The overall mean recovery was 81%.

Scientific Data Images for Rat IL-2 ELISA Kit (Colorimetric)

ELISA: Rat IL-2 ELISA Kit (Colorimetric) [NBP1-92704]

Kit Contents for Rat IL-2 ELISA Kit (Colorimetric)

  • 1 vial (2.2 ml) monoclonal Coating Antibody to rat IL-2 (100 ug/ml)
  • 1 vial (22 ul) Streptavidin-HRP
  • 1 vial (55 ul) Biotin-Conjugate anti- rat IL-2 monoclonal antibody
  • 1 vial rat IL-2 Standard protein lyophilized, 40 ng/ml upon reconstitution
  • 2 vials (50 ml) Sample Diluent

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-2

Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors in an autocrine fashion on the surface of T cells. Apart from its most important role to mediate antigen specific T lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B and NK cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T cells to synthesize IL-2 have been observed in physiologic and pathologic states.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Rat IL-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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