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Rat IL-3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06776

Novus Biologicals, part of Bio-Techne
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NBP3-06776

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat IL-3 in samples. No significant cross-reactivity or interference between Rat IL-3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 6.06

Inter-Assay Precision (Precision between assays) 5.45

Recovery for Rat IL-3 ELISA Kit (Colorimetric)

Recovery

92 - 108 %

Linearity

Rat IL-3 ELISA Kit (Colorimetric)

Scientific Data Images for Rat IL-3 ELISA Kit (Colorimetric)

Rat IL-3 ELISA Kit (Colorimetric)

ELISA: Rat IL-3 ELISA Kit (Colorimetric) [NBP3-06776] -

ELISA: Rat IL-3 ELISA Kit (Colorimetric) [NBP3-06776] - Standard Curve Reference

Kit Contents for Rat IL-3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-3

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for Rat IL-3 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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