Human IL-4R alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80348

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Cell Supernatant (100 ul)

Sensitivity

2.6 pg/ml

Assay Range

3.125-200 pg/ml (example only; lot dependent)

View all IL-4R alpha ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human IL-4R alpha.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.4

Inter-Assay Precision (Precision between assays) CV% < 5.7 %

Recovery for Human IL-4R alpha ELISA Kit (Colorimetric)

Recovery

serum (n=3): 89 -97%
supernatant (n=3): 87 -96%

Kit Contents for Human IL-4R alpha ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: IL-4R alpha

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of na ve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Gene Symbol

IL4R

Additional IL-4R alpha Products

Product Documents for Human IL-4R alpha ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-4R alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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