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Mouse Insulin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62853

Novus Biologicals, part of Bio-Techne
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NBP2-62853

Key Product Details

Sample Type & Volume Required Per Well

cell culture supernatant and tissue homogenate (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31-20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.00

Inter-Assay Precision (Precision between assays) CV% < 10.00

Recovery for Mouse Insulin ELISA Kit (Colorimetric)

Recovery

95-110%

Linearity

Mouse Insulin ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Insulin ELISA Kit (Colorimetric)

Mouse Insulin ELISA Kit (Colorimetric)

ELISA: Mouse Insulin ELISA Kit (Colorimetric) [NBP2-62853] -

ELISA: Mouse Insulin ELISA Kit (Colorimetric) [NBP2-62853] - Standard Curve Reference

Kit Contents for Mouse Insulin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100×)
  • Concentrated HRP Conjugate (100×)
  • Concentrated Wash Buffer (25×)
  • HRP Conjugate Diluent
  • Micro ELISA Plate(Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents for Mouse Insulin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Insulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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